You Searched For: Cloning Buffers

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.

In molecular biology, cloning buffer solutions containing TRIS, acetic acid, EDTA, or more are used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Various buffer formulations are available with different base ingredients in the mixture. These different mixtures have varied buffer capacities and run times. Buffer solutions serve to protect samples against pH changes while the sample is undergoing a wide variety of chemical applications.


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Catalog Number: (45001-155)
Supplier: Corning
Description: Tris-EDTA (TE) buffer is commonly used in work with nucleic acids.

Catalog Number: (EM-3700)
Supplier: MilliporeSigma
Description: Cloning Buffer

Supplier: Promega Corporation
Description: This EDTA solution is a chelator of divalent cations and is suitable for biochemistry and molecular biology applications
Catalog Number: (IB70185)
Supplier: IBI Scientific
Description: EDTA Solution is commonly used in biological and electrophoresis buffer systems.

SDS


Catalog Number: (PAC6711)
Supplier: Promega Corporation
Description: The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors. Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer.

Catalog Number: (77462-178)
Supplier: AAT BIOQUEST INC
Description: ReadiUse™ Universal Kinase Reaction Stopping Buffer is an aqueous solution that effectively chelates divalent metal ions.


Supplier: Promega Corporation
Description: TE Buffer, 1X, Molecular Grade (pH 8.0), is a buffer composed of 10 mM Tris-HCl containing 1 mM EDTA.Na<sub>2</sub>.
Catalog Number: (AAJ63240-AP)
Supplier: Thermo Scientific Chemicals
Description: Liquid

Catalog Number: (AAJ62848-AK)
Supplier: Thermo Scientific Chemicals
Description: Liquid

Catalog Number: (101227-398)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

Small Business Enterprise


Catalog Number: (PAC1313)
Supplier: Promega Corporation
Description: Designed for use with T4 Polynucleotide Kinase to catalyze the transfer of the γ-phosphate from ATP to the 5´-terminus of poly- or mononucleotides bearing a 5´-hydroxyl group.

Catalog Number: (AAJ60832-AK)
Supplier: Thermo Scientific Chemicals
Description: 17076431

Supplier: Teknova
Description: RNAse, DNAse Tested (10mM Tris, 0.1mM EDTA)
Catalog Number: (102855-118)
Supplier: New England Biolabs (NEB)
Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes

Small Business Enterprise


Catalog Number: (80058-050)
Supplier: MilliporeSigma
Description: 17076431

Supplier: Thermo Scientific Chemicals
Description: Sodium acetate buffer solution pH, 5.2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis. Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as Chip-on-Chip assay and in vitro enzyme inhibition assay.
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