Pierce 1-Step™ NBT/BCIP Substrate Solution, Thermo Scientific

Supplier: 0000013509

34042
PI34042EA 242.85 USD
PI34042
Pierce 1-Step™ NBT/BCIP Substrate Solution, Thermo Scientific
Electrophoresis Reagents Western Blotting Reagents

The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.


  • Sensitive with low background and minimal assay-to-assay variability
  • Ready-to-use single component


The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.

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