NEBNext Ultra II FS DNA PCR-free Library Prep Kit with Sample Purification Beads, New England Biolabs

Supplier: New England Biolabs (NEB)

E7435S E7435L
76538-644EA 680 USD
76538-644 76538-646
NEBNext Ultra II FS DNA PCR-free Library Prep Kit with Sample Purification Beads, New England Biolabs
Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of intact DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.


  • ncludes NEBNext FS (Fragmentation System) enzymatic fragmentation reagents
  • Save time: optimized PCR-free workflow, reduced hands-on time and automation compatibility
  • Generate high quality libraries without an amplification step with inputs from 50 to 500 ng
  • Improved library uniformityIncludes SPRIselect® size selection and clean-up beads for added flexibility and reliability


The fast, user-friendly workflow includes enzymatic fragmentation for optimal input size, requires minimal hands-on time, and maintains excellent library yields and GC coverage without requiring PCR. This kit includes DNA sample purification beads for added convenience. Don’t be biased! The NEBNext® Ultra™ II FS DNA PCR-free Library Prep Kit for Illumina® can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs®, you can trust that your samples are in the best hands – your own.

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