Luna® Probe One-Step RT-qPCR 4X Mix with UDG, New England Biolabs

Supplier: New England Biolabs (NEB)

M3019S M3019L M3019X M3019E
76459-512EA 356 USD
76459-512 76459-514 76459-516 76459-518
Luna® Probe One-Step RT-qPCR 4X Mix with UDG, New England Biolabs
Nucleic Acid Reagents qPCR/RT-qPCR Enzymes and Kits

The Luna® Probe one-step RT-qPCR 4X mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes.


  • Simplify your reaction setup with a single-tube master mix format
  • Increase sensitivity of your RT-qPCR, as 4X concentration allows for more sample input
  • Maximize your throughput by multiplexing up to five targets
  • Reduce risk of carryover contamination, with UDG and dUTP included in the mix
  • Eliminate pipetting errors with non-interfering, visible blue tracking dye


In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.

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