LipidSpot™ 610 lipid droplet stains
Supplier: Biotium
LipidSpot™ stains are fluorescent dyes that rapidly stain lipid droplets in live or fixed cells, with no wash step and minimal background.
- Rapidly and specifically stain lipid droplets
- Minimal background, no wash required
- Stain live or fixed cells, or fix and permeabilize after staining
- Available with green or red/far-red fluorescence
- Supplied at 1000X in DMSO
Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. LipidSpot™ dyes are fluorogenic neutral lipid stains that rapidly accumulate in lipid droplets, where they become brightly fluorescent. The dyes can be used to stain lipid droplets in both live and fixed cells, with no wash step required. Cells also can be fixed and permeabilized after staining. LipidSpot™ stains show minimal background staining of cellular membranes or other organelles, unlike traditional dyes like Nile Red
LipidSpot™ 610 has excitation/emission at ~592/638 nm in cells; it is optimally detected in the Texas Red® channel, but is also bright in the Cy®3 and far-red Cy®5 channels. Therefore, we don’t recommend pairing LipidSpot™ 610 with other red or far-red probes.
In yeast, LipidSpot™ 488 stains intracellular membranes, but LipidSpot™ 610 does not. In bacteria, both LipidSpot™ dyes can stain gram-positive but not gram-negative strains. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Specification Test Results
Abs/Em | 610/663 nm (in vegetable oil) ~592/638 nm (in cells) |
Detection channel | Texas Red® or Cy®5 |
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