Biocytin TMR, CF® 594 Dye
Supplier: Biotium
Synonyms:
Biocytin conjugates
, 5-(&-6)-Tetramethylrhodamine Biocytin
CF®594, Truly the brightest deep red dye
- Yields the brightest antibody conjugates among spectrally similar dyes.
- Excellent choice for multicolor imaging with green dyes like CF®488A
- Extremely photostable
- Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy
CF® dye biocytin is a cell-impermeant fixable polar tracer that is a conjugate of CF® dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.
Stock solutions may be prepared in DMF or DMSO.
CF® dye biocytin is available in the following colors: CF®488A, CF®568, CF®594, and CF®640R.
Caution: Store at –20 °C, protected from light.
Specification Test Results
Abs/Em Maxima | 593/614 nm |
Extinction coefficient | 115,000 |
Molecular weight | ~730 |
Excitation laser line | 532 nm, 568 nm or 594 nm |
Replaces | Alexa Fluor® 594, DyLight® 594, Texas Red® |
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