Biocytin TMR, CF® 488A Dye
Supplier: Biotium
Synonyms:
Biocytin conjugates
, 5-(&-6)-Tetramethylrhodamine Biocytin
CF®488A is a superior green fluorescent dye
- Minimally charged, for less non-specific binding than Alexa Fluor® 488
- Narrower emission spectrum for less bleed to red
- Very photostable
- Compatible with super-resolution imaging by TIRF
- Highly water soluble and pH-insensitive
CF® dye biocytin is a cell-impermeant fixable polar tracer that is a conjugate of CF® dye and biotin with an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis.
Stock solutions may be prepared in DMF or DMSO.
CF® dye biocytin is available in the following colors: CF®488A, CF®568, CF®594, and CF®640R.
Caution: Store at –20 °C, protected from light.
Specification Test Results
Abs/Em Maxima | 490/515 nm |
Extinction coefficient | 70,000 |
Molecular weight | ~914 |
Excitation laser line | 488 nm |
Replaces | Alexa Fluor® 488, DyLight® 488, fluorescein (aka FITC, FAM), Cy®2 |
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