MPure Bacterial DNA Extraction Kit is used with the MPure-12 Nucleic Acid Purification System for extraction and purification of genomic DNA from both Gram-positive and Gram-negative bacteria.
- Extraction of genomic DNA from Gram-positive and Gram-negative bacteria
- Starting materials include bacterial pellet/colony from culture, cell-free body fluids, liquid transport media, urine, and environmental material
- Completely automated purification protocol
- Use with MPure-12 System
- Run in as little as 55 minutes
- Nucleic Acids can be used immediately in downstream applications
MPure™ Bacterial DNA Extraction Kit contains everything needed for sample preparation. However, for tough samples such as Gram+ bacteria or spores, soil or feces, sample preparation using FastPrep™ Instruments and Lysing Matrix Tubes can greatly increase yields as well as shorten processing time.
Capacity
Bacterial Colony: 200-400 µL /1-3 colony, Bacterial Pellet: 200-400 µL /Up to 109 bacteria (about OD600 = 3), cell-Free body fluids: 200-400 µL cell-free body fluids, Liquid Transport media: 200-400µL liquid transport media, Tissue: 200-400 µL /1-30 mg, Urine: 200-400 µL /5-50 mL urine
Packaging: Contains Proteinase K solution = 40 µL, Lysis Buffer 2 = 720 µL, Binding Buffer 1 = 720 µL, Magnetic Bead Solution = 800 µL, Washing Buffer 1 = 1000 µL, Washing Buffer 2 = 1000 µL, Washing Buffer 3 = 1000 µL, Elution Buffer 1 = 1000 µL, Elution Buffer 2 = 1000 µL
Caution: Store at room temperature (15-25°C). Do not freeze the reagent cartridges. The Kits are stable for 12 months under these conditions. Store the purified DNA at 4°C (short-term) or aliquot and store at –70°C (long-term) before performing the downstream analysis.
